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. Author manuscript; available in PMC: 2023 Feb 14.
Published in final edited form as: Blood Rev. 2020 Jul 10;46:100732. doi: 10.1016/j.blre.2020.100732

Table 2:

Suggested Antibody/Fluorophore Panels Used for Two Flow Cytometry Assays.

MSKCC Ten-color MFC EuroFlow Eight-Color MFC
Single Tube Tube 1 (cell-surface markers) Tube 2 (cell-surface and cytoplasmic markers)
CD117 PC5.5 CD117 APC
CD19 PC7 CD19 PC7 CD19 PC7
CD138 APC CD138 BV421 CD138 BV421
CD56 APC-R700 CD56 PE CD56 PE
CD45 APC-H7 CD45 PerCP-Cy5.5 CD45 PerCP-Cy5.5
CD81 Pacific Blue CD81 APC-C750
CD38 BV510 CD38 FITC CD38 FITC
CD27 BV605 CD27 BV510 CD27 BV510
κ FITC κ APC
λ PE λ APC-C750

Selection of recommended antibody-fluorochrome combinations used for two sensitive flow cytometric MRD assays capable of detection levels to 10−6 [31–33].