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. 2023 Feb 13;12(1):2174721. doi: 10.1080/2162402X.2023.2174721

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — SBRT determines an increase of central-memory and effector-memory T cells. (a-c) Dot-plots showing CCR7 versus CD45RA staining, evaluated by multicolor flow cytometry, in gated CD8⁺ (a) and CD4⁺ (c) T cells from two representative patients, during and after RT. (b-d) Pooled results from 18 patients (Group1-3FRX, n = 5; Group2-20FRX, n = 6; Group3-30FRX, n = 7), showing the proportion of central-memory (CM, CCR7⁺CD45RA⁻), naïve (CCR7⁺CD45RA⁺), effector-memory (EM, CCR7⁻CD45RA⁻) and terminally-effector (EMRA, CCR7⁻CD45RA⁺) T cells, in gated CD8⁺ (b) and CD4⁺ (d) T cells (left panels) (Wilcoxon U-test). Right panels, comparison among Groups (Mann-Whitney U-test). *p ≤ .05. Abbreviation: FRX = fraction; MP = Middle point; POST = 6 months after RT.