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. 2023 Feb 8;14(1):2171636. doi: 10.1080/21505594.2023.2171636

Figure 1.

Figure 1.

Resistin secreted by PAMs damages monolayer PAECs integrity. (a, b) H. parasuis (100 MOI) infected wild-type or resistin knockout PAMs. PAMs lysates were taken 2 h after infection. The resistin expression was determined using western blot analysis (A), and the mRNA resistin level was analysed using qRT-PCR (B). The antibodies against resistin and β-actin were diluted at 1/1000 and 1/50000, respectively. β-actin served as a loading control, and the relative resistin levels of wild-type cells were calculated with ImageJ software and normalized to β-actin. **p<0.01 compared with the uninfected wild-type group. (c) Wild-type or resistin knockout PAMs were co-cultured with PAECs. TEER of PAECs was measured at 0, 6, 12, 24, 48, and 2 h after PAMs were infected with H. parasuis (100 MOI). The TEER levels were displayed as a percentage of the TEER before treatment. The mean ± SEM (n = 3) is presented as a representative result of three independent tests. **p<0.01 compared with the wild-type group.