Table 1.

Plasmids used in this study.

Plasmids	Characteristics	Source
pK18mobsacB	Suicide vector, Kan resistance	ATCC 87,097
pK18-UKD	Kan fragment, upstream and downstream sequences of relative genes in pK18mobsacB, Kan resistance	This study
pSHK3	E. coli - H. parasuis shuttle vector, Kan resistance	[20]
pSHK3-Gm	Kan is replaced by Gm in pSHK3, Gm resistance	[20]
pSHK3-C-lppA	A fragment containing the promoter and complete lppA ORF in pSHK3-Gm, Gm resistance	This study
pDG459	SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs	Addgene plasmid #100901