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. 2023 Feb 7;2023:8934210. doi: 10.1155/2023/8934210

Figure 2.

Figure 2

SAMMSON silencing inhibits melanoma cell proliferation in vitro. (a) The knockdown efficiency of the ASO-mediated suppression of SAMMSON in A375 cells was detected by RT-qPCR. (b, c) Cell growth curves of SAMMSON knockdown and control melanoma cells were plotted using MTT assays. The horizontal coordinate represents the time of cell culture, and the ordinate represents the absorbance value of the cell at the wavelength of 490 nm. (d, e) Evaluation of the proliferative capacity of SAMMSON-silenced and control melanoma cells using the colony formation assay. (f–j) RT-qPCR analysis of the relative mRNA expression of caspase 3, Bcl-2, CDK2, CDK4, and P27 in SAMMSON-silenced and control melanoma cells. (k, l) Western blotting analysis of the protein expression of CDK4 and cyclin D1 in SAMMSON-silenced and control melanoma cells. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. control cells.