Figure 3.

SAMMSON silencing inhibits melanoma cell migration and invasion in vitro. (a, b) Analysis of the effect of SAMMSON knockdown on melanoma cell migration and invasion using Transwell assays. Representative images of migrated and invaded cells are shown in the top panel; quantitative results are shown below the images. (c–g) RT-qPCR analysis of the relative mRNA expression of E-cadherin, vimentin, N-cadherin, MMP9, and MMP2 in SAMMSON-silenced and control melanoma cells. (h, i) Western blotting analysis of the protein expression of MMP9 and MMP2 in SAMMSON-silenced and control melanoma cells. Scale bars = 100 μm. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001 vs. control cells.