Figure 7.

FOXA2 inhibits melanoma cell proliferation in vitro. (a) Cell growth curves of NC-OE and FOXA2-OE melanoma cells were plotted using MTT assays. (b) The proliferative capacity of NC-OE and FOXA2-OE melanoma cells was determined by plate clone formation assays. (c) Flow cytometry analysis of the cell cycle of NC-OE and FOXA2-OE melanoma cells. (d–f) RT-qPCR analysis of the relative mRNA expression of CDK2, CDK4, and cyclin D1 in NC-OE and FOXA2-OE melanoma cells. (g–i) Western blotting analysis of the protein expression of CDK4, P27, and P53 in NC-OE and FOXA2-OE melanoma cells. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001 vs. control cells.