Extended Data Fig. 4. Cytosolic STARD7 is required for CoQ transport from the mitochondria to the plasma membrane.
a, Immunoblot analysis of cellular fractions obtained by differential centrifugation of lysates of WT and cyto-STARD7 overexpressing cells. The highlighted fractions 8 K and 40 K represent mitochondrial fraction and plasma membrane (PM) fractions, respectively, and were used to measure CoQ and other lipids. WC, whole cell; PNS, post-nuclear supernatant, n = 2 independent biological experiments. b, Raw data representation of 4 independent fractionation experiments from Fig. 4g showing CoQ10 levels measured by mass spectrometry in 8,000xg (8 K) and 40,000xg (40 K) pellet.