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. 2023 Jan 19;25(2):246–257. doi: 10.1038/s41556-022-01071-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Scheme showing the fractionation protocol for HeLa cells to isolate mitochondria and PM fractions with differential centrifugations (image created with BioRender.com). b,c, Heat maps showing the distribution of mitochondrial (b) and plasma membrane (c) proteins in different fractions of cells determined by MS. #1, 8,000g pellet; #2, 12,000g pellet; #3, 40,000g pellet and #4, 100,000g pellet. n = 5 biologically independent experiments. d,e, Total levels of CoQ10/9 measured in indicated fractions of HeLa cells. CoQ distribution within the cell is indicated in per cent in each fraction. f,g, Differences in the abundance of CoQ10, CoQ9, PC, PE (f,g) and sphingomyelin (SM) (g) in mitochondrial and plasma membrane fractions relative to WT in STARD7^−/− cells and STARD7^−/− cells complemented with either mito-STARD7 or cyto-STARD7 (f) and in WT cells overexpressing cyto-STARD7 (g). In d–g, data were analysed by Instant Clue software and are represented by 95% confidence interval of the mean. n = 4 (d,e,g) and n = 5 (f) biologically independent experiments. In d–g, the P values were calculated using a two-tailed Student’s t-test for unpaired comparisons (d,e) and two-tailed one-sample t-test for unpaired comparison (f and g). Source numerical data are available in source data.

Source data