Extended Data Fig. 2. PARL and STARD7 protect cells against ferroptosis.
a,b, |Cell death measurement after 24 h (a) or kinetics (b) in WT cells treated with indicated concentrations of erastin (a) or combination of erastin (3 µM) and PUFA (arachidonic acid 20:4, 40 µM) (a,b), in the presence of ferroptosis inhibitors Fer1 (1 µM) or CoQ2 (1 µM) and apoptosis inhibitor QVD (1 µM) as indicated. WT cells treated with DMSO were used as control (CT). c, Cell death kinetics in WT and PARL−/− cells treated with erastin (3 µM) and PUFA (40 µM). d,e, Immunoblot analysis of WT, PARL−/− (d) and STARD7−/− (e) human colorectal cancer HCT116 cells. #1 and #2 represent two different monoclonal cell lines of the indicated genotype. a, n = 4 and b−c, n = 3 biological independent experiments. d−e, Representative immunoblots of n = 2 independent experiments. a−c, Data is represented by 95% confidence interval of the mean and the P values (a) were calculated using a two-tailed Student’s t-test for unpaired comparisons.