Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 16;25(2):351–365. doi: 10.1038/s41556-022-01064-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 7 — (a) Balloon-plot of known neuronal and glial cell markers (SOX10-MKI67). Progenitor: SOX10¹²³, FOXD3¹²⁴, ASCL1⁴², Neuronal: PHOX2B¹²⁵, PRPH¹²⁶, NRG1¹²⁷, TUBB3¹²⁸, Sympathetic neurons: DBH, TH¹²⁹, Parasympathetic neurons: NOS1, VIP¹³⁰, Sensory neurons: PRDM12, P2RY1, TRPV1^131,132, Schwann Cell Progenitors (SCPs): CDH19, MPZ, PLP1¹³³, Glial cells: GFAP, S100B^134,135, Chromaffin cells: PNMT, PENK, CARTPT¹³⁶ and Proliferating cells: MKI67¹¹⁴, PCNA¹²⁰. The remaining genes correspond to the top-5, most selective genes for each cluster. From the statistically significant genes (adjusted p-value < 0.001, MAST with Bonferroni correction using all features), the top-10 (log2 fold-change) were sorted according to the percent of positive cells and the top-5 were plotted. Gene order follows the cluster order. Balloon size: percent of positive cells. Color intensity: scaled expression. Blue: high, Gray: low. (b) Balloon-plot of the detected cholinergic-synapse pathway genes (KEGG id: 217716). Balloon size: percent of positive cells. Color intensity: log₂(normalized UMI-counts+1) (library size was normalized to 10.000) expression. Blue: high, Gray: low. (c) Heatmap of differentially expressed transcription factors (TFs) along the SCP-neuronal trajectory, according to tradeSeq²¹. Stars: analyzed genes in ‘D-E’. Color intensity: scaled expression. Dark red: high, Gray: low. (d) UMAP-plots of SOX10, ASCL1 and ISL1 TFs. Expression levels: log₂(normalized UMI-counts+1) (library size was normalized to 10.000). Blue: high. Gray: zero. (e) Confocal-microscopy image of an 8.5 pcw ganglion, showing SOX10, ASCL1 and ISL1 expression, detected with immunofluorescence. Dashed outlines: manually segmented nuclei. SOX10^pos SCPs (arrows), SOX10^pos ASCL1^pos transitioning SCPs (asterisks), ASCL1^pos SOX10^neg immature neurons (hashes), ISL1^pos ASCL1^neg mature neurons (arrowheads). Scale-bar: 5 µm.