TABLE 3.
Alive/dead status of G. mellonella waxworm larvae, and presence of microbial proliferation as scored through GFP fluorescence status of the waxworm, over 3 days post-injection with various strains of Arsenophonus, with E. coli MG1655 known to be not capable of proliferation, and BHI medium injection as controls.
| Day 1 | Day 2 | Day 3 | |||||||
| Galleria alive bacteria present | Galleria alive bacteria absent | Galleria dead | Galleria alive bacteria present | Galleria alive bacteria absent | Galleria dead | Galleria alive bacteria present | Galleria alive bacteria absent | Galleria dead | |
| A. nasoniae aNv_FIN | 0 | 25 | 0 | 0 | 25 | 0 | 0 | 25 | 0 |
| A. nasoniae aPv | 0 | 25 | 0 | 0 | 25 | 0 | 0 | 25 | 0 |
| Arsenophonus sp. aPb | 20 | 5 | 0 | 23 | 2 | 0 | 23 | 2 | 0 |
| E. coli MG1655 | 0 | 20 | 5 | 0 | 20 | 5 | 0 | 20 | 5 |
| BHI control | 0 | 20 | 0 | 0 | 20 | 0 | 0 | 20 | 0 |
N=25 for all cases except BHI control, N=20. Galleria alive bacteria present, waxworm alive with signs of bacterial proliferation as assessed by GFP fluorescence; Galleria alive bacteria absent, waxworm alive without signs of bacterial proliferation as assessed by GFP fluorescence; Galleria dead, waxworm had died.