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. 2023 Feb 1;14:1089143. doi: 10.3389/fmicb.2023.1089143

TABLE 3.

Alive/dead status of G. mellonella waxworm larvae, and presence of microbial proliferation as scored through GFP fluorescence status of the waxworm, over 3 days post-injection with various strains of Arsenophonus, with E. coli MG1655 known to be not capable of proliferation, and BHI medium injection as controls.

Day 1 Day 2 Day 3
Galleria alive bacteria present Galleria alive bacteria absent Galleria dead Galleria alive bacteria present Galleria alive bacteria absent Galleria dead Galleria alive bacteria present Galleria alive bacteria absent Galleria dead
A. nasoniae aNv_FIN 0 25 0 0 25 0 0 25 0
A. nasoniae aPv 0 25 0 0 25 0 0 25 0
Arsenophonus sp. aPb 20 5 0 23 2 0 23 2 0
E. coli MG1655 0 20 5 0 20 5 0 20 5
BHI control 0 20 0 0 20 0 0 20 0

N=25 for all cases except BHI control, N=20. Galleria alive bacteria present, waxworm alive with signs of bacterial proliferation as assessed by GFP fluorescence; Galleria alive bacteria absent, waxworm alive without signs of bacterial proliferation as assessed by GFP fluorescence; Galleria dead, waxworm had died.