. 2023 Jan 27;415(7):1357–1369. doi: 10.1007/s00216-023-04527-8

Table 2.

Precursor and product pairs and associated retention time and collision energies

Vitamin D metabolite and adduct	Precursor	Products	Retention time (min)	Collison energy (V)
1,25(OH)₂D₃ [M+H-H₂O]⁺	399.3	381.2	7.02	9.0
1,25(OH)₂D₃ [M+H-H₂O]⁺	399.3	135.1	7.02	21.0
1,25(OH)₂D₂ [M+NH₄]⁺	446.3	411.3	7.36	9.0
1,25(OH)₂D₂ [M+NH₄]⁺	446.3	135.1	7.36	11.0
1,25(OH)₂D₃-d₃ [M+NH₄]⁺	437.3	384.3	7.03	8.0
1,25(OH)₂D₃-d₃ [M+NH₄]⁺	437.3	138.1	7.03	17.0
24,25(OH)₂D₃ [M+H]⁺	417.3	381.3	6.48	8.0
24,25(OH)₂D₃ [M+H]⁺	417.3	399.3	6.48	7.0
24,25(OH)₂D₂ [M+H-2H₂O]⁺	393.3	268.1	7.02	24.0
24,25(OH)₂D₂ [M+H-2H₂O]⁺	393.3	243.2	7.02	9.0
24,25(OH)₂D₃-d₆ [M+H]⁺	423.3	405.2	6.47	9.0
24,25(OH)₂D₃-d₆ [M+H]⁺	423.3	387.2	6.47	13.0
25(OH)D₃ [M+H]⁺	401.30	383.3	8.98	7.0
25(OH)D₃ [M+H]⁺	401.30	365.3	8.98	8.0
25(OH)D₂ [M+H]⁺	413.3	395.3	9.20	8.0
25(OH)D₂ [M+H]⁺	413.3	107.2	9.20	21.0
25(OH)D₃-c₅ [M+H]⁺	406.2	388.2	8.97	8.0
25(OH)D₃-c₅ [M+H]⁺	406.2	370.2	8.97	7.0