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Lower limit of detection (LLOD), lower limits of quantification (LLOQ), and linear range of exogenous standards, using a maximum of ten measuring points between 1 fg/mL and 10 ng/mL
| Vit-D metabolite | LLOD (pg/mL) | LLOQ (pg/mL) | Linear range (pg/mL) |
|---|---|---|---|
| 1,25(OH)2D3 | 1 | 12.5 | 1–1000 |
| 1,25(OH)2D2 | 1 | 12.5 | 10–1000 |
| 24,25(OH)2D3 | 1 | 12.5 | 10–1000 |
| 24,25(OH)2D2 | 1 | 12.5 | 10–1000 |
| 25(OH)D3 | 1 | 12.5 | 10–1000 |
| 25(OH)D2 | 1 | 12.5 | 10–1000 |
