Table 5.

Intra-day accuracy and precision (RSD%)

Concentration, pg/mL	Vitamin D metabolite	Day 1		Day 2		Day 3
		Accuracy, RSD%	Precision, RSD%	Accuracy, RSD%	Precision, RSD%	Accuracy, RSD%	Precision, RSD%
100	1,25(OH)2D3	2.35	3.97	−16.96	4.23	5.89	6.04
	1,25(OH)2D2	−9.37	2.29	8.18	7.25	−9.58	0.82
	24,25(OH)2D3	3.56	4.40	4.15	7.23	10.57	6.65
	24,25(OH)2D2	−6.76	5.11	−6.76	4.77	12.93	11.33
	25(OH)D3	3.51	3.49	4.29	13.89	11.04	2.82
	25(OH)D2	14.23	11.73	4.76	22.32	17.66	3.35
500	1,25(OH)2D3	−12.06	9.59	−9.70	1.00	−6.83	4.33
	1,25(OH)2D2	−8.81	2.79	7.25	6.4	−12.12	2.73
	24,25(OH)2D3	−17.35	1.88	9.40	4.20	−4.53	1.03
	24,25(OH)2D2	−16.68	1.93	−16.68	2.96	6.81	1.77
	25(OH)D3	−6.80	5.51	14.69	7.73	−3.87	2.05
	25(OH)D2	−3.52	4.52	13.84	5.45	−2.51	3.21
1000	1,25(OH)2D3	12.87	11.64	−3.84	0.98	5.61	16.10
	1,25(OH)2D2	8.67	7.08	−15.97	6.04	−15.01	19.50
	24,25(OH)2D3	−1.39	5.44	17.66	22.05	−31.57	4.13
	24,25(OH)2D2	−0.41	5.37	−0.41	4.38	10.44	6.80
	25(OH)D3	2.36	7.00	14.91	4.03	−18.02	16.33
	25(OH)D2	12.90	7.58	18.23	5.16	−2.11	22.06