Table 7.

Recovery difference (%) of metabolites for the prespike and postspike extraction procedure in acetonitrile matrix showing mean, SD, and RSD (%)

		1,25(OH)2D2	1,25(OH)2D3	24,25(OH)2D3	24,25(OH)2D2	25(OH)D2	25(OH)D3
Prespike	Mean	5.32	0.15	2.17	1.73	1.32	11.89
	SD	0.31	0.00	0.08	0.05	0.04	0.50
	RSD%	5.75	2.20	3.53	3.15	3.34	4.18
Postspike	Mean	5.76	0.15	2.32	1.71	1.36	12.71
	SD	1.54	0.04	0.28	0.32	0.43	1.33
	RSD%	26.73	28.44	11.99	18.78	31.69	10.47
	Recovery % (postspike/prespike)*100	108.32	105.39	106.60	99.09	103.01	106.92