Fig. 13.

Schematic of AsFlFFF stages. a The sample is injected and focused between the two opposing flows (parabolic channel flow and reverse flow) in the channel. In the elution stages, the parabolic channel from inlet to outlet caused the sequential elution of particles of different sizes. b The particles reach heights depending on their diffusion coefficient (normal mode). c For larger particles, diffusion is negligible and their position in the channel depends on physical size (steric mode). Reprinted by permission from [27], Springer Nature, Nature Protocols, Copyright 2019