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. 2022 Oct 26;415(7):1265–1285. doi: 10.1007/s00216-022-04362-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 14 — Schematic of an on-line coupled immunoaffinity chromatography-asymmetric-flow field-flow fractionation system for analysis of nano-sized biomolecules from plasma. The system is comprised of a a monolithic column for immunoaffinity chromatography, b an automated six port valve for injection to AsFlFFF, c AsFlFFF with ultraviolet, multiangle light-scattering, dynamic light-scattering, and diode array detectors, and d a fraction collector. Figure reprinted from [28]