FIG 1.

Mutation spectrum in liquid biopsies from patients with GEP-NEC. The oncoplot shows the most frequently altered genes (rows) in liquid biopsies from 50 patients with GEP-NEC (columns). Upper panel shows the mutational burden per sample, with colors indicating mutation type for individual mutations. Percentages on the right represent mutation frequency per gene, with colors indicating mutation types (and a separate, purple color for multiple mutations in the same gene in the same patient). The panel under the oncoplot area is composed of three single-row heatmaps showing in order, from top to bottom, primary tumor site, cell type, and MSI status. Multihit indicates that more than one mutation occurs in the same gene, in the same patient. Mutations presented represent the findings in liquid biopsies after additional filtering against WBC-data (see Methods). GEP-NEC, gastroenteropancreatic neuroendocrine carcinoma, MSI, microsatellite instable; TMB, tumor mutation burden.