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. 2023 Feb 14;14:836. doi: 10.1038/s41467-023-36271-z

Fig. 5. TN1 affects the expression of abscisic acid (ABA) biosynthetic gene TaNCED3 and ABA content in the shoot base and tiller bud.

Fig. 5

a, b Morphology of the shoot base (a) and tiller buds (b) used for RNA-seq and ABA/IAA contents analysis. The tiller buds are indicated with red lines. Scale bars, 1 mm in (a), 2 cm in (b). c Heatmap representation of the expression levels of DEGs in the shoot base and tiller buds shared between YZ4110 and the tn1 mutant. d RT-qPCR analysis showing the expression patterns of TaNCED3 genes in YZ4110 and the tn1 mutant. TaNCED3-5B, TraesCS5B02G029300; TaNCED3-5D, TraesCS5D02G038800. The relative expression levels were normalized to TaGAPDH. Data are means ± SD of six biological replicates. e, f Measurement of ABA and indole-3-acetic acid (IAA) contents in the shoot base and tiller buds of the tn1 mutant, respectively. Data are means ± SD of three biological replicates. The p values in d–f are indicated by two-tailed unpaired t-test. Source data are provided as a Source Data file.