Fig. 4. Therapy-induced evolution of monocyte-like DC and cycling T cell immune phenotype.

a Histogram plot showing cell proportions of myeloid cell clusters in the G3 (n = 12 samples) and G4 Groups (n = 5 samples) colored by cell type. DC, dendritic cell; Macro, macrophage; Mono, monocyte. Point colors correspond to samples. Data are presented as mean values ± SEM (error bars); p-values are calculated by two-sided unpaired Student’s t-test. b Split violin plots showing the antigen-presenting, pro-angiogenic, and proliferative function scores of myeloid cells in the G3 (n = 12 samples, red) and G4 Groups (n = 5 samples, blue) groups. Box represents median ± interquartile range; p-values are calculated by two-sided unpaired Wilcoxon test. c Gene Set Enrichment Analyses (GSEA) analysis showing distinct enrichment pathways of C3-DC in the G3 (red) and G4 Groups (blue). Bar chart showing normalized enrichment scores (NES) of specific pathways. d Heatmap plot representing the activity of metabolism pathways of C2/C3-DCs in the G3 and G4 Groups. Color indicates the activity score of each metabolism pathway calculated by Gene Set Variation Analysis (GSVA) analysis. e Histogram plot representing the cell proportion of T/NK clusters in the G3 (n = 12 samples) and G4 Groups (n = 5 samples), colored by cell type. Point colors correspond to samples. Data are presented as mean values ± SEM (error bars); p-values are calculated by two-sided unpaired Student’s t-test. f Split violin plots showing the cytotoxic, inhibitory, naïve, and proliferative function scores of T cells in the G3 (n = 12 samples, red) and G4 Groups (n = 5 samples, blue). Box represents median ± interquartile range; p-values are calculated by two-sided unpaired Wilcoxon test. g Similar to d, the metabolism pathway activity score of C3-CD4 and cycling T cells in the G3 and G4 Groups shown in a heatmap plot. Pathway activity scores were calculated by Gene Set Variation Analysis (GSVA) analysis. Source data are provided as a Source Data file.