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. 2023 Feb 14;14:822. doi: 10.1038/s41467-023-36310-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Cell proportions of myeloid cell clusters after Chemotherapy (n = 3 samples) and Immunotherapy (n = 2 samples) shown in histogram plot. DC, dendritic cell; Macro, macrophage; Mono, monocyte. Histogram colors correspond to cell clusters; point colors correspond to samples. Data are presented as mean values ± SEM (error bars). b Dotplot showing the antigen-presenting, pro-angiogenic, and proliferative function scores of myeloid cell clusters in Chemotherapy (n = 3 samples) and Immunotherapy (n = 2 samples) Groups. Dot size represents percent of expressing cells in each cluster, and color represents mean expression level of selected gene signatures. c, d Volcano plots showing differentially expressed genes (DEGs) of C2-DC (c) and C3-DC (d) between Chemotherapy (three patients) and Immunotherapy (two patients) Groups. e Split violin plots showing expression levels of immune checkpoints of C2/C3-DC and C3-Macro in Chemotherapy (n = 3 samples, red) and Immunotherapy (n = 2 samples, blue) Groups. The comparisons and statistical analyses are conducted between cell clusters (three cell clusters: C2-DC, C3-DC, and C3-Macro) of Chemotherapy and Immunotherapy groups, and the total cells number of all clusters are >3. Box represents median ± interquartile range; p-values are calculated by two-sided unpaired Wilcoxon test. f The same histogram plot as in a for T/NK clusters in Chemotherapy (n = 3 samples) and Immunotherapy (n = 2 samples) Groups. Histogram colors correspond to cell clusters; point colors correspond to samples. Data are presented as mean values ± SEM (error bars). g Dotplot showing the cytotoxic, naïve, and proliferative function scores of T/NK cell types in Chemotherapy (three patients) and Immunotherapy (two patients) Groups. Dot size represents percent of expressing cells in each cluster, and color represents z-score of normalized mean expression level of selected gene signatures. h The same split violin plots as in (e) for T/NK cell types in Chemotherapy (n = 3 samples, red) and Immunotherapy (n = 2 samples, blue) Groups. The comparisons and statistical analyses are conducted between T cell clusters (10 cell clusters: C1-3 CD4, C1-6 CD8, and Cycling T) of Chemotherapy and Immunotherapy groups, and the total cells number of all clusters are >3. Box represents median ± interquartile range; p-values are calculated by two-sided unpaired Wilcoxon test. Source data are provided as a Source Data file.