Skip to main content
. 2022 Dec 18;10(5):2202976. doi: 10.1002/advs.202202976

Figure 4.

Figure 4

Chemogenetic stimulation promotes myelin repair in mice. a) Heatmaps generated from DTI axial views of FA acquired from sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. b) Quantification of FA values in the corpus callosum in the sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. The measured values were normalized to the mean value of the sham group. n = 11 in the sham mCherry group, n = 20 in the BCAS mCherry group and n = 19 in the BCAS hM3D group. c) Representative images of Black‐gold staining and immunostaining of MBP and MAG in the corpus callosum in the sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. Scale bar: 200 µm. Quantification of d) Black‐gold staining and immunostaining of e) MBP and f) MAG in the corpus callosum in the sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. n = 4 per group. g) Immunoblot bands and quantification of h) MBP and i) MAG expression in the corpus callosum in the sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. The intensity of each immunoblot band was normalized to that of the β‐actin band. The measured values were normalized to the mean value of the sham group. n = 4 per group. j) Representative TEM images of the corpus callosum in the sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. Scale bar: 1 µm. k) The percentage of myelinated axons in the corpus callosum in the sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. n = 4 per group. l) Scatterplots of the myelin g‐ratio as a function of the axon diameter in the corpus callosum in the sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. Axons were selected from 4 mice per group for measurement. n = 104, n = 110, and n = 117 measured axons for each group. m) Representative curves of CAPs of myelinated N1 fibers in the corpus callosum in the sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. n) Quantification of the amplitude of evoked CAPs of myelinated N1 fibers in the corpus callosum in the sham mCherry, BCAS mCherry, and BCAS hM3D groups at 2 months after surgery. n = 3 mice and 14–17 recordings per group. The data are presented as the mean ± SEM. p‐values were determined by the Kruskal–Wallis test with Dunn's post‐hoc analysis in (b) and (l); by 1‐way ANOVA with Tukey's post‐hoc analysis in (d), (e), (f), (h), (i), and (k); and by 2‐way ANOVA with Tukey's post‐hoc analysis in (n). *p < 0.05, **p < 0.01, ***p < 0.001.