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Evaluation of the ability of nanometers to cross the BBB. A) Simulation of the BBB model in vitro. B) The CLSM images of U87MG cells incubated with ODP‐TH, PpIX, and Dox for 4 h in transwell and fluorescence semi‐quantitative statistics (C,D). Fluorescence of Dox in orthotopic glioma model at different time (E). Mean ± SD, n = 3. p‐values were indicated by *** (p < 0.001). Scale bar = 20 µm.
