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Regeneration of positive tobacco plants and PCR identification of Kan resistance. (A) Kanamycin medium for bud differentiation. (B) Resistant plant culture. (C) Rooting of resistant plants. (D) Transplanted seedling culture. (E) Transgenic tobacco identification using genomic PCR, 1~7: resistant regenerated tobacco, W: negative control, -: blank control, +: positive control, M: DL2000 DNA marker.
