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. 2023 Jan 10;299(2):102891. doi: 10.1016/j.jbc.2023.102891

Figure 2.

Figure 2

Molecular weight and oligomeric state of the recombinant neuraminidases.A, graphs showing the size-exclusion chromatography (SEC) elution profiles of the (upper panel) recombinant N1 and N2 proteins and (lower panel) enzymatic activities. Arrowheads indicate the elution profile of the molecular weight standards. Activities are from equal volumes of the 200 μl fractions collected between 4 and 8.8 ml. B, graphs displaying the SEC elution profiles of the (upper panel) recombinant NanA, NanB, and NanC proteins and (lower panel) enzymatic activities. SEC elution profiles of the molecular weight standards (arrowheads) are depicted. Activities are from equal volumes of 200 μl fractions collected between 4 and 9.8 ml. C, SEC-MALS chromatograms of the (left panel) recombinant N1 and N2 and the (right panel) recombinant NanA, NanB, and NanC are displayed. For each protein the absorbance (Abs) reading at 280 nm is plotted along with the in-line molecular weight (MW) measurements obtained by multiangle light scattering (MALS).