Figure 5.

Stability variation between the neuraminidases.A, thermostability of (left panel) N1 and N2 and (right panel) NanA, NanB, and NanC was determined at each pH by performing a melt curve analysis that used enzymatic activity as a readout and calculating the T₅₀. Data for each protein are from three independent biological replicates. B, urea stability of (left panel) N1 and N2 and (right panel) NanA, NanB, and NanC was assessed by measuring the enzymatic activity at each pH in the presence of increasing concentrations of urea in three independent experiments. The urea concentration corresponding to 50% inhibition of activity is displayed from each experiment. p values were generated using a one-way ANOVA Dunnett’s multiple comparisons test with a 95% CI, using pH 7 as the comparator. ∗∗∗∗p ≤ 0.0001, ∗∗∗p ≤ 0.001, ∗∗p ≤ 0.01, ∗p ≤ 0.05, ns p > 0.05.