Table 2.
Neuraminidase apparent and true catalytic rates for MUNANA in each pH buffer
| pH | Apparent kcats-1 |
True kcats-1a |
|||||
|---|---|---|---|---|---|---|---|
| N1 | N2 | NanA | NanB | NanC | N1 | N2 | |
| 4 | 10 ± 2 | 1 ± 0 | 10 ± 1 | 0 ± 0 | 0 ± 0 | 53 ± 9 | 5 ± 0 |
| 5 | 10 ± 2 | 18 ± 7 | 170 ± 4 | 100 ± 18 | 14 ± 1 | 54 ± 10 | 77 ± 29 |
| 6 | 31 ± 4 | 58 ± 3 | 344 ± 6 | 228 ± 13 | 38 ± 1 | 163 ± 20 | 253 ± 12 |
| 7 | 30 ± 4 | 69 ± 4 | 202 ± 53 | 91 ± 13 | 12 ± 2 | 160 ± 20 | 300 ± 16 |
| 8 | 13 ± 1 | 30 ± 1 | 55 ± 8 | 13 ± 1 | 2 ± 0 | 67 ± 3 | 132 ± 5 |
| 9 | 4 ± 0 | 15 ± 1 | 9 ± 1 | 1 ± 0 | 0 ± 0 | 22 ± 2 | 66 ± 3 |
a
Values were adjusted based on the fraction of active N1 and N2 in preparations measured by TR1.