Figure 1.

Examples of available backbone plasmids for constructing shuttle vectors for A. platensis transformation

(A–C) (A) pDV002, a streptomycin-selectable plasmid targeting the NS1 site in the A. platensis genome (B) pDV044, a kanamycin-selectable plasmid targeting the KmR-modified region (as described in Jester et al.¹) in the A. platensis genome. The plasmid contains the validated promoter region P_cpc600 (C) pDV052, a kanamycin-selectable plasmid targeting the KmR-modified region in the A. platensis genome. “Left arm” is the 5′ homologous arm of the region of insertion; “right arm” is the 3′ homologous arm of the region of insertion. BsaI and BbsI are restriction sites available for Golden Gate cloning. “aadA” is the streptomycin marker for A. platensis. “KmR” is the kanamycin selection marker for A. platensis. “AmpR” is the ampicillin marker for selection in E. coli. “p15a ori” is a low-copy origin of replication in E. coli and “colE1 ori” is a high-copy origin of replication in E. coli.