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. 2023 Feb 8;4(1):102087. doi: 10.1016/j.xpro.2023.102087

Figure 7.

Figure 7

A. platensis transformation and assessment process

Strains are transformed with a plasmid carrying a gene of interest flanked by homologous arms for integration into the genome. The cells whose genomes contain the integrated transgene are able to survive antibiotic selection. These surviving strains can then be assessed for segregation, protein expression, frozen and grown for any required purpose.