10×SOT
| Reagent | Final concentration | Amount |
|---|---|---|
| Deionized water | N/A | About 990 mL |
| K2HPO4 (Potassium phosphate dibasic) | 28.6 mM | 5 g |
| NaNO3 (Sodium nitrate) | 294 mM | 25 g |
| K2SO4 (Potassium sulfate) | 57.3 mM | 10 g |
| NaCl (Sodium chloride) | 171 mM | 10 g |
| MgSO4 (Magnesium sulfate) | 8.12 mM | 0.977 g |
| CaCl2⋅2H2O (Calcium chloride dihydrate) | 2.7 mM | 0.4 g |
| FeSO4⋅7H2O (Iron (II) sulfate heptahydrate) | 0.35 mM | 0.1 g |
| Na2EDTA⋅2H2O (Sodium ethylenediaminetetraacetic acid dihydrate) | 2.15 mM | 0.8 g |
| A5 solution | N/A | 10 mL |
| Total | 1 L |
Store at 4 C for up to one month.