REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
Arthrospira platensis UTEX LB 1926	Culture Collection of Algae at the University of Texas at Austin	www.utex.org
Chemicals, peptides, and recombinant proteins
Proteinase K powder (≥30 U/mg)	Thermo Fisher Scientific	Cat# 17916
RNAse A powder, DNAse free	Fisher Scientific	Cat# 50-153-8124
Lysozyme	VWR	Cat# 97062-138
Cetyltrimethylammonium bromide	Fisher Scientific	Cat# ICN19502905
Sodium bicarbonate	VWR	Cat# 144-55-8
Boric acid	Fisher Scientific	Cat# AC423485000
Manganese sulfate monohydrate	Thermo Fisher Scientific	Cat# A17615.36
Zinc sulfate heptahydrate	Thermo Fisher Scientific	Cat# A12915.36
Copper (II) sulfate pentahydrate	VWR	Cat# BDH9312-500G
Sodium molybdate dihydrate	Millipore Sigma	Cat# M1003-100G
Potassium phosphate dibasic	Millipore Sigma	Cat# P8281-100G
Sodium nitrate	Thermo Fisher Scientific	Cat# 014493.30
Potassium sulfate	Thermo Fisher Scientific	Cat# A13975.0I
Sodium chloride	Thermo Fisher Scientific	Cat# 012314.A9
Magnesium sulfate	Thermo Fisher Scientific	Cat# 413485000
Calcium chloride dihydrate	VWR	Cat# BDH9224-1KG
Iron (II) sulfate heptahydrate	Thermo Fisher Scientific	Cat# A15178.0E
Sodium ethylenediaminetetraacetic acid dihydrate	Millipore Sigma	Cat# ED4SS-100G
Hydrochloric acid 6 N	Millipore Sigma	Cat# XX0628-01
Sodium hydroxide 10 N	Millipore Sigma	Cat # SX0607N-6
Sodium hydroxide pellets	Millipore Sigma	Cat # S5881-500G
Molecular grade water	Fisher Scientific	Cat# AAJ71786K8
Tris base	Fisher Scientific	Cat# BP152-500
Tris HCl	Millipore Sigma	Cat# T5941-100G
SDS (sodium dodecyl sulfate)	Millipore Sigma	Cat# 11667289001
Glycerol	Fisher Scientific	Cat# BP229-1
Ammonium acetate	Fisher Scientific	Cat# AAA1634330
Kanamycin sulfate	Fisher Scientific	Cat# BP906-5
Streptomycin sulfate salt	Millipore Sigma	Cat# S6501-5G
G418 Sulfate, powder	Fisher Scientific	Cat# MT61234RF
Molecular grade isopropanol	Fisher Scientific	Cat# BP2618500
Molecular grade ethanol (200 proof)	Fisher Scientific	Cat# BP2818500
Non-sterile isopropyl alcohol, 70%	Fisher Scientific	Cat# 19-130-713
Sodium acetate 3 M, pH 5.2	Fisher Scientific	Cat# 56-742-2100ML
Linear acrylamide, 5 mg/mL	Fisher Scientific	Cat# NC1781917
Chloroform isoamyl alcohol mixture, 24:1	Fisher Scientific	Cat# 11-101-6907
SapphireAmp Master Mix	Takara	Cat# RR350B
KAPA HiFi PCR Kit	Roche	Cat# KK2101
Critical commercial assays
Miniprep Kit	Qiagen	Cat# 27106
NEBridge Golden Gate Assembly Kit	NEB	Cat# E1601S
Deposited data
Arthrospira platensis YZ	Xu et al.2	www.ncbi.nlm.nih.gov Taxonomy ID: 1738638
Recombinant DNA
pDV002	This paper	Addgene 191823
pDV044	This paper	Addgene 191824
pDV052	This paper	Addgene 191825
Other
Phase-lock tube, heavy	VWR	Cat# 10847-802
Laminar flow hood in good working condition with a UV irradiating sterilization lamp and aspirator	Any brand/model available in the lab	N/A
A light meter to determine light intensity	Any brand/model available in the lab. Example used by authors in “identifier” column	Licor LI-250A light meter
Aliquoting pipetteman	Any brand/model available in the lab. Example used by authors in “identifier” column	Vistalab ali-Q 2 VS with variable aliquoting speed
Standard pipetteman	Any brand/model available in the lab	N/A
Microcentrifuge	Any brand/model available in the lab. Example used by authors in “identifier” column	Eppendorf 5425
Tabletop centrifuge that can accommodate 50 and 15 mL conical tubes	Any brand/model available in the lab. Example used by authors in “identifier” column	Eppendorf 5810R
Standard 1,000, 200 and 20 μL pipettors	Any brand/model available in the lab	N/A
1.5 mL microcentrifuge tube-compatible racks	Any brand/model available in the lab	N/A
14 mL round bottom tube-compatible racks	Any brand/model available in the lab	N/A
Sterile 50- and 250-mL flasks	Any brand/model available in the lab	N/A
Sterile 0.5, 1 and 2 L bottles	Any brand/model available in the lab	N/A
Spray bottle for holding the cleaning solution (70% isopropanol)	Any brand/model available in the lab	N/A
An adjustable-light shaker with ambient CO2 that can accommodate 14 mL plastic round-bottom tubes and flasks	Any brand/model available in the lab. Example used by authors in “identifier” column	New Brunswick Innova 4400 with programmable illumination up to 1,500 μE
An adjustable-light shaker with supplemental CO2 that can accommodate 14 mL plastic round-bottom tubes and flasks	Any brand/model available in the lab. Example used by authors in “identifier” column	Infors Multitron Pro shaker-incubators with programmable illumination up to 1,500 μE
Thermomixer	Any brand/model available in the lab. Example used by authors in “identifier” column	Eppendorf Thermomixer C with 2.0 mL, 1.5 mL, 50 mL attachments
DNA fluorometer	Any brand/model available in the lab. Example used by authors in “identifier” column	Qubit Flex Fluorometer
DNA fluoremetry kit	Any brand/model available in the lab. Example used by authors in “identifier” column	Qubit 1× dsDNA High Sensitivity (HS) Kit
Vacufuge	Any brand/model available in the lab. Example used by authors in “identifier” column	Eppendorf Vacufuge Plus Concentrator
Sterile 1,000, 200 and 10 μL filtered pipette tips	Any brand/model available in the lab	N/A
Sterile 14 mL plastic round-bottom tubes (ex: Corning Falcon round-bottom polypropylene test tubes)	Any brand/model available in the lab. Example used by authors in “identifier” column	Corning Falcon round-bottom polypropylene test tubes
Sterile 50 mL, 25 mL, 10 mL and 5 mL serological pipettes	Any brand/model available in the lab	N/A
Sterile 1.5 mL clear microcentrifuge tubes (avoid colored plastics for more consistency of light penetration)	Any brand/model available in the lab	N/A
Sterile 50 mL and 15 mL conical tubes	Any brand/model available in the lab	N/A
Sterile 1,000, 200 and 10 μL filtered pipette tips	Any brand/model available in the lab	N/A
Filter sterilization system for bottles, 0.22 μM or smaller	Any brand/model available in the lab	N/A
Positive-displacement pipette, 5 mL	Any brand/model available in the lab	N/A
Kimwipes	Fisher Scientific	Cat#06-666