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5% β-Mercaptoethanol solution
| Reagent | Final concentration | Amount |
|---|---|---|
| dH2O | N/A | 246 μL |
| 1 M Tris-HCl (pH 7.5) | 120 mM | 36 μL |
| β-Mercaptoethanol 100% | 5% | 15 μL |
| Triton X-100 100% | 1% | 3 μL |
| Total | N/A | 300 μL |
Note on storage conditions: should be freshly prepared right before use.
