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. 2023 Feb 8;25:239–255. doi: 10.1016/j.bioactmat.2023.01.024

Fig. 5.

Fig. 5

Osteogenesis enhancement induced by Sr-MBGNs scaffolds promoted NCPs secretion and Kindlin-2/PTH1R/OCN axis upregulation. a) GO enrichment analysis of osteogenesis function; b) Volcano plots indicating the differentially expressed upregulated and downregulated genes for Sr-MBGNs and GelMA group; c) The related upregulated genes collected from the GO enrichment of osteogenesis function presented in the heatmap; d) Schematic of the NCPs regulation mechanism (schematic created with BioRender.com); e) Representative Western blot analysis verifying the upregulation of the Kindlin-2/PTH1R/OCN axis at the protein level; f) KEGG pathway analysis screening the most correlated signaling pathways; g) IHC indicating the positive correlation between the expression level of OCN and PTH1R at 2 w and 4 w with HE staining used to display the bone defect region; h) immunofluorescent staining confirming the high expression level of Kindlin-2 in TIID BMSCs after 7 d incubation with 15Sr and 30Sr-MBGNs groups; i) immunofluorescent staining indicating the positive correlation between OCN and PTH1R for TIID BMSCs after 7 d incubation with the scaffolds.