Figure 4.

Relative mRNA expression in the liver. The Chicken β-actin was served as the housekeeping gene. Expression of IL-6 (A), TNF-α (B), TLR4 (C), and NF-κB p65 (D) mRNA were assessed by real-time quantitative PCR. A relative quantitative method (2^−ΔΔCt) was employed to evaluate the quantitative variation. Data are represented as means ± SE (n = 6). In each histogram, bars sharing different letters are significantly different (P < 0.05).