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. 2022 Nov 9;42(4):e112275. doi: 10.15252/embj.2022112275

Figure 2. Dfm1 colocalizes and binds to SPOTS complex proteins.

Figure 2

  1. Dfm1‐GFP binding to Lcb2‐RFP and Orm2‐RFP was analyzed by co‐IP. As a negative control, cells not expressing Dfm1‐GFP were used (3 biological replicates; n = 3).
  2. Dfm1‐GFP colocalizes with Lcb1‐RFP and Orm22‐RFP. Strains were grown to mid‐exponential phase in minimal media GFP, and RFP fluorescence was examined on an AxioImager.M2 fluorescence microscope using a 100x objective and 28HE‐GFP or 20HE‐rhodamine filter sets (3 biological replicates; n = 3; Zeiss). Scale bar, 5 μm.