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. 2022 Nov 9;42(4):e112275. doi: 10.15252/embj.2022112275

Figure EV4. Orm2‐3A accumulates exclusively in the ER. Related to Fig 7 .

Figure EV4

  1. Fluorescence imaging was performed as in Fig 7B except for WT and dfm1∆ cells expressing Orm2‐3A‐GFP was used. Sec61‐RFP (ER marker, red) was used to test for co‐localization with Orm2‐3A‐GFP (2 biological replicates; n = 2). Arrowheads indicate Orm2 co‐localizing in post‐ER compartments. Scale bar, 5 μm.
  2. dfm1∆ cells do not abrogate COPII‐mediated export of CPY. The indicated cells were either grown at room temperature or shifted to non‐permissive growth at 37°C. Cells were analyzed by SDS–PAGE and immunoblotted for CPY with α‐CPY and PGK1 with α‐PGK1.
  3. Western blot of aggregated versus soluble Orm2‐GFP at the ER. Lysates from WT and dfm1Δ cells containing ORM2‐GFP were blotted using anti‐GFP to detect Orm2. P is ER aggregated fraction and S is ER soluble fraction.