Reagent	Source	Identifier
Antibodies
Anti‐IDH2 monoclonal antibody in mouse	Abcam	Cat#: ab55271; RRID: AB_943793
Purified Mouse Anti‐Human Hif1‐α monoclonal antibody in mouse	Santa Cruz Biotechnologies	Cat#: sc‐13515; RRID: AB_627723
Carbonic anhydrase IX monoclonal antibody in mouse	BioScience	Cat# DF13218; RRID: AB_2846237
Anti‐GLUT1 (A‐4) monoclonal antibody in mouse	Santa Cruz Biotechnologies	Cat# sc‐377228; RRID: AB_2190936
Anti‐TIGAR polyconal antibody in rabbit	Invitrogen	Cat# PA1‐41487; RRID: AB_2259272
PKM2 (D78A4) XP Rabbit monocloncal antibody in rabbit	Cell Signaling Technology	Cat# 4053; RRID: AB_1904096
LDHA‐Specific polyclonal antibody in rabbit	Proteintech	Cat# 199871‐1‐AP
Anti‐ G6PD imonocloncal antibody in mouse	Santa Cruz Biotechnologies	Cat# sc‐373886; RRID: AB_10918100
Anti‐TKT polyclonal antibody produced in rabbit	Sigma‐Aldrich	Cat#HPA029481; RRID: AB_10558885
Anti‐CTPS1 (416‐430) polyclonal antibody produced in rabbit	Sigma‐Aldrich	Cat# SAB1101072; RRID: AB_10606733
Anti‐PHD2 polyclonal antibody in rabbit	Invitrogen	Cat# PA1‐16525; RRID: AB_568563
Ki‐67, mouse monoclonal anibody	Dako	# Cat: M7240
Anti‐β‐actin monoclonal antibody in mouse	Sigma‐Aldrich	#Cat: A5441
Chemicals
Cisplatin	Nippon Kayaku	N/A
Gemcitabine	Nippon Kayaku	N/A
2'‐Deoxycytidine	Sigma‐Aldrich	#Cat: D3897
AGI6780	Selleck Chemicals	#Cat: S7241
AGI221 (Enadesinib)	Cayman Chemical	#Cat: 21277
[13C5]‐glutaime	Millipore Sigma	#Cat: 605166
G6PD‐inhibitor	Selleck Chemicals	#Cat: S9617
Leflunomide	Selleck Chemicals	#Cat: S1247
Mizoribine	Selleck Chemicals	#Cat: S1384
BPTES	Selleck Chemicals	#Cat: S7753
2'‐Deoxyadenosine monohydrate	Selleck Chemicals	#Cat: S9351
2'‐Deoxyguanosine monohydrate	Selleck Chemicals	#Cat: S9007
Thymidine	Selleck Chemicals	#Cat: S4803
Galiiac acid	Selleck Chemicals	#Cat: S4603
Digoxin	Selleck Chemicals	#Cat: S4290
Buthionine Sulfoximine	Sigma‐Aldrich	#Cat: 415383
Critical commercial assays
Blood & Cell Culture DNA extraction Kits	Qiagen	#Cat: 13323
Cell Tox Green Cytotoxicity Assay	Promega	#Cat: G8741
NADP/NADPH Quantitation Colorimetric Kit	Biovision	#Cat: K347‐100
CellROX® Orange	Thermo Fischer Scientific	#Cat: C10443
GSH‐Glo Glutathione	Promega	#Cat: V6911
Dharmafect transfection Reagent 1	Thermo Fischer Scientific	#Cat: T‐2001
Xfect™ Transfection Reagent	Takara Bio	631317Z1317N
pcDNA™3.1 (+) Mammalian Expression Vector	Invitrogen	V79020
Seahorse XF Glycolytic Rate Assay Kit	Agilent Technologies	103344‐100 XF
Seahorse XFe24 FluxPak mini	Agilent Technologies	102342‐100
Seahorse XF RPMI assay medium pack	Agilent Technologies	103681‐100
Experimental models: cell lines
Human: T24	American Type Culture Collection	#Cat: ATCC HTB‐4
Human: UMUC3	American Type Culture Collection	#Cat: ATCC CRL‐1749
Human: J82	American Type Culture Collection	#Cat: ATCC HTB‐1
Experimental models: oraganisms/strains
BALB/cAJcl‐nu/nu	CLEA Japan, Inc.	#Cat: 6496
Oligonucleotides
Taqman Probe hIDH2	Applied Biosystems	Hs00953879_m1
Taqman Probe hEGLN1	Applied Biosystems	Hs00254392_m1
Taqman Probe hHIF1A	Applied Biosystems	Hs00153153_m1
Taqman Probe hGLUT1	Applied Biosystems	Hs00892681_m1
Taqman Probe hG6PD	Applied Biosystems	Hs00166169_m1
Taqman Probe hCTPS1	Applied Biosystems	Hs01041852_m1
Taqman Probe hTIGAR	Applied Biosystems	Hs00608644_m1
Taqman Probe hTKT	Applied Biosystems	Hs0115545_m1
Taqman Probe hPKM2	Applied Biosystems	Hs00987255_m1
Taqman Probe hLDHA	Applied Biosystems	Hs03405707_g1
Taqman Probe hDHODH	Applied Biosystems	Hs00361406_m1
Taqman Probe hUMPS	Applied Biosystems	Hs00923517_m1
Taqman Probe hCTPS2	Applied Biosystems	Hs00219845_m1
Taqman Probe hTYMS	Applied Biosystems	Hs00426586_m1
Taqman Probe hIMPDH1	Applied Biosystems	Hs04190080_gH
Taqman Probe hATIC	Applied Biosystems	Hs00269671_m1
Taqman Probe hPFAS	Applied Biosystems	Hs00389822_m1
Taqman Probe hATIC	Applied Biosystems	Hs00269671_m1
Taqman Probe hPRPS1	Applied Biosystems	Hs00751338_s1
Taqman Probe hACTB	Applied Biosystems	Hs01060665_g1
Recombinant DNA
siRNA‐hIDH2 target sequence #1	Thermo Fischer Scientific	s227
siRNA‐hIDH2 target sequence #2	Thermo Fischer Scientific	106706
Silencer siRNA Non‐targeting control	Invitrogen	#Cat:AM4611
siRNA‐hHIF1A target sequence #1	Thermo Fischer Scientific	s6539
siRNA‐hHIF1A target sequence #2	Thermo Fischer Scientific	s6541
Software and Algorithms
GraphPad Prism 8 software	Graph Pad	N/A
Statistical Package of Social Sciences software ver 24.0	IBM	N/A