Figure EV4. Nitarsone prevents Aβ‐induced cAMP‐responsive element‐binding protein (CREB) shutoff in vitro .

• A–C
  Acute treatment with 10 μM Nitarsone rescuses Aβ_1‐42‐induced CREB shutoff. (A) Scheme of the experimental design. The dissociated, hippocampal cell cultures at DIV16 were either pre‐treated for 30 min with 10 μM Nitarsone and subsequently 2 h with 500 nM Aβ_1‐42 or the drug was added 2 h after the 500 nM Aβ_1‐42 treatment. The pCREB immunoreactivity was measured in comparison to vehicle control. (B) Representative confocal images of hippocampal neurons. Lookup table indicates the pixel intensities from 0 to 255. Scale bar: 10 μm. (C) Bar plot representing nuclear pCREB immunostaining intensity normalized to vehicle control. N = 88–101 from 5 to 7 independent cell cultures. ***P < 0.001 by two‐way ANOVA with Sidak's post hoc test.
• D
  Treatment with 1 μM TTX induced upregulation of GluR1 surface expression. N = 21–23 dendritic segments from three independent cell cultures. **P < 0.01 by two‐tailed Student's t‐test.

Data information: All data are represented as mean ± SEM.

Source data are available online for this figure.