Figure 8.

Effects of PS-MPLs on Bax and Bcl-2 expression in the ovary and apoptosis in GCs in rats

(A–E) Immunohistochemical analysis of Bcl-2 and Bax expression (A). The various groups in Fig A are the control group (a), PS-MPLs 0.015 (b), PS-MPLs 0.15 (c), and PS-MPLs 1.5 mg/d (d). Bcl-2 and Bax expressions’ IOD values were determined by the Image-pro plus 6.0 (B and C). Apoptotic rate (D) and percent apoptotic rate (E) in GCs after PS-MPLs treatment were determined by flow cytometer using PI- and FITC-labeled Annexin V double staining. Adopted with permission from Elsevier.¹⁰²