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. 2023 Jan 9;11(2):41. doi: 10.21037/atm-22-6063

Figure 5.

Figure 5

PRDX6 ameliorates HG-triggered apoptosis and the inflammatory response via TLR4/NF-κB signaling. (A) Effect of TLR4 activator CRX-527 or NF-κB activator PMA on the viability of the PRDX6-overexpressing HK-2 cells in a HG environment were examined by CCK-8 assays. (B,C) TUNEL assays were used to examine the effect of CRX-527 or PMA on the apoptosis of PRDX6-overexpressing HK-2 cells in a HG environment. Magnification, 200×. Western blot analysis results of the expression of (D) apoptosis-related factors and (E) inflammatory factors. +P<0.05, ++P<0.01, +++P<0.001 vs. Control group; ***P<0.001 vs. HG group; ##P<0.01, ###P<0.001 vs. HG + Oe-PRDX6 group. HG, high glucose; TLR4, toll-like receptor 4; NF-κB, nuclear factor-kappa B; PMA, phorbol 12-myristate 13-acetate; HK-2, human kidney 2; CCK-8, Cell Counting Kit-8; TUNEL, terminal-deoxynucleotidyl transferase mediated nick end labeling.