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. 2023 Jan 31;11(2):52. doi: 10.21037/atm-22-1121

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2023 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Effect of AP on adipogenic differentiation of BMSCs from osteoporotic mice. (A) Representative images of ORO staining of lipid droplets of BMSCs in the presence of AP (×200); (B) quantification analysis of ORO staining; (C-E) relative levels adipogenic gene markers: Pparg (C), Fabp4 (E) and LPL (E), were measured by qRT-PCR in BMSCs induced to differentiate into adipocytes for 16 days. Data are presented as mean ± SD. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. OVX, ovariectomy; AP, Andrographis paniculata; BMSCs, bone marrow mesenchymal stem cells; Fabp4, fatty acid binding protein 4; Pparg, peroxisome proliferator activated receptor-g; LPL, lipoprotein lipase; ORO, Oil Red O; qRT-PCR, quantitative real-time polymerase chain reaction.