Table 1. Primers used for qRT-PCR amplification.
Gene | Primer sequence (5' to 3') |
---|---|
Runx2 (mouse) | F: ACTTCCTGTGCTCCGTGCTG |
R: TCGTTGAACCTGGCTACTTGG | |
Osterix (mouse) | F: ACCAGGTCCAGGCAACAC |
R: GCAAAGTCAGATGGGTAAGTAG | |
ALP (mouse) | F: CGTCTCCATGGTGGATTATGC |
R: TGGCAAAGACCGCCACAT | |
Pparg (mouse) | F: GACCACTCGCATTCCTTT |
R: CCACAGACTCGGCACTCA | |
Fabp4 (mouse) | F: AAATCACCGCAGACGACA |
R: CACATTCCACCACCAGCT | |
LPL (mouse) | F: GGGAGTTTGGCTCCAGAGTTT |
R: TGTGTCTTCAGGGGTCCTTAG | |
β-actin (mouse) | F: CTGTCCCTGTATGCCTCTG |
R: TGATGTCACGCACGATTT |
qRT-PCR, quantitative real-time polymerase chain reaction; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; Pparg, peroxisome proliferator activated receptor-g; Fabp4, fatty acid binding protein 4; LPL, lipoprotein lipase.