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. 2023 Jan 31;11(2):52. doi: 10.21037/atm-22-1121

Table 1. Primers used for qRT-PCR amplification.

Gene Primer sequence (5' to 3')
Runx2 (mouse) F: ACTTCCTGTGCTCCGTGCTG
R: TCGTTGAACCTGGCTACTTGG
Osterix (mouse) F: ACCAGGTCCAGGCAACAC
R: GCAAAGTCAGATGGGTAAGTAG
ALP (mouse) F: CGTCTCCATGGTGGATTATGC
R: TGGCAAAGACCGCCACAT
Pparg (mouse) F: GACCACTCGCATTCCTTT
R: CCACAGACTCGGCACTCA
Fabp4 (mouse) F: AAATCACCGCAGACGACA
R: CACATTCCACCACCAGCT
LPL (mouse) F: GGGAGTTTGGCTCCAGAGTTT
R: TGTGTCTTCAGGGGTCCTTAG
β-actin (mouse) F: CTGTCCCTGTATGCCTCTG
R: TGATGTCACGCACGATTT

qRT-PCR, quantitative real-time polymerase chain reaction; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; Pparg, peroxisome proliferator activated receptor-g; Fabp4, fatty acid binding protein 4; LPL, lipoprotein lipase.