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Paxillin FRAP in ZMEL cells in vitro. Related to Fig. 2. Example of a FRAP video of a ZMEL Paxillin-EGFP cell in the in vitro cell culture conditions. A single Paxillin-positive punctum (as indicated by the red circle) is bleached, and fluorescence recovery after bleaching is recorded as a single Z-plane. Images were taken every 2 s for 5 min, 7 fps.
