Table 2.
Primers used in this study.
| Primer pairs | Oligonucleotide sequences (5′-3′)* | Purposes |
|---|---|---|
| hns-F1-up- BamHI | GCGGGATCCTTCCACAATTCATTGGCATCAC | Δhns deletion strain construction |
| hns-F1-dn | ATCCAAATTGTGAACAGGAATTTTGCCAGA | |
| hns-F2-up | TGAACAGGAATTTTGCCAGAAACTAAAATG | |
| hns-F2-dn-SpeI | GGACTAGTACACCGAAGATTCCGCTAAAC | |
| fis-F1-up- BamHI | GCGGGATCCGGTGAGGCGGAATACGACAG | Δfis deletion strain construction |
| fis-F1-dn | ACGTCGGTGAAGAATTCGGTCTAGCTCTTC | |
| fis-F2-up | GAAGAGCTAGACCGAATTCTTCACCGACGT | |
| fis-F2-dn-SpeI | GGACTAGTAAAGTGGGCGAGTAGGGTTTC | |
| hns-Tn7-NotI-up | GCGCGGCCGCTCAAGCGACATCATGTCAAC | C7258Δhns::hns complementation strain construction and identification |
| hns-Tn7-XhoI-dn | GCTCTAGATCAGTATCCGTTCGAGTTAA | |
| glms-F | CGATTGCGGTAGAAGCGTC | |
| glms-hnsR | AGACTAAATGAGCCAAATGA | |
| thyA-qPCR-up | ACATGGGACGCGTGTATGG | qPCR for thyA |
| thyA-qPCR-dn | ATATGACCACCATCAGGCTTAGC | |
| rfbT-qPCR-up | TTCTTGAAAGCGAATTTGGATTGC | qPCR for rfbT |
| rfbT-qPCR-dn | GTGTATATGACGAGCAGCGATTC | |
| rfbT1-up-SacI | CCCGAGCTCCGCAACAGAGCAAG ATGT | Construction of rfbT-lux reporter plasmids |
| rfbT2-up-SacI | CCCGAGCTCTTAGAGCGGACGATCGAG | |
| rfbT-dn-BamHI | CGGGATCCGACTGAATAGCATCAAGC | |
| rfbT-hns-shift-up | CAAGGATCAGGCAGATATG (5’biotin label) | Probe-hns |
| rfbT-hns-shift-dn | CTTGCAGATGCAGGTTTGAG (5’biotin label) | |
| rfbT-crp-shift-up | CGTTACTTGAAGCGACTTGT(5′ biotin-labeled) | Probe-N7 |
| rfbT-crp-shift-dn | CAAACATATCTGCCTGATCC (5′ biotin-labeled) | |
| rfbT-up (FAM) | CAAGGATCAGGCAGATATG | DNase I footprinting assay |
| rfbT-dn | CTTGCAGATGCAGGTTTGAG | |
| rfbT1-M1-R | GGGTTCGCTCTGTGTGAGGTTCAAACA | Construction of mutant rfbT-lux reporter plasmids |
| rfbT1-M1-F | TGTTTGAACCTCACACAGAGCGAACCC | |
| rfbT1-M3-1-R | AATGGATTTGCCATGTGTGTGACATTTAGAAG | |
| rfbT1-M3-1-F | CTTCTAAATGTCACACACATGGCAAATCCATT | |
| rfbT1-M3-2-R | GATTTGCCATTTTAGTTCCATTTAGAAG | |
| rfbT1-M3-2-F | CTTCTAAATGGAACTAAAATGGCAAATC |
*The underlined bases indicate the restriction enzyme sites.