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引物序列
| 基因 | 序列5′-3′ | |
| ALP | F: GAAGGAGGCAGGATTGAC | R: ATCAGCAGTAACCACAGTC |
| OCN | F: AGTCTGACAAAGCCTTCA | R: AAGCAGGGTTAAGCTCACA |
| COL-Ⅰ | F: CAGTGGCGGTTATGACTT | R: CTGCGGATGTTCTCAATCT |
| BMP-2 | F: TGTGAGGATTAGCAGGTCTT | R: TCGTTTGTGGAGCGGATG |
| BMP-4 | F: ATTGGCTCCCAAGAATCATGG | R: CGTGATGGAAACTCCTCACAGT |
| Smad 1 | F: CTCACATGCCACTGAACGCC | R: TCCGCATACACCTCTCCTCC |
| Smad 5 | F: TTTGCAGAGCCATCACGAGC | R: TTTCCAATATGCCGCCTAGT |
| β-actin | F: GATTACTGCTCTGGCTCCT | R: ACTCCTGCTTGCTGATCC |
