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. 2023 Feb 15;8:19. doi: 10.1038/s41541-023-00620-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — IFNγ secreting spot forming cells (SFC) were enumerated in BAL (a–e) and spleen (f–j) on D83. Cells were stimulated with a pool of peptides covering NP (a, f), NA2 (b, g) and M1 (c) proteins or pH1N1 (d, i) and H3N2 (e, j) viruses. Each symbol represents an individual animal, the top of the bar the mean and the line the standard error (SEM). Asterisks denote significance between indicated groups (*p < 0.05, **p < 0.01, ****p < 0.0001) and were analyzed either by one-way ANOVA and the Bonferroni multiple comparisons test when the data were normally distributed (a–c, f, h, i) or with Kruskal–Wallis and Dunn’s multiple comparisons test when normality was not achieved (d, e, g, j).