Table 3.
Antibodies used for the intracellular cytokine staining.
| Antigen | Clone | Isotype | Fluorochrome | Dilution used | Source of primary Ab | Details of secondary Ab |
|---|---|---|---|---|---|---|
| CD4 | 74-12-4 | IgG2b | PerCP-Cy5.5 | 1:50 | BD Biosciences (Cat. No: 561474) | |
| CD8b | PPT23 | IgG1 | FITC | 1:200 | Bio-Rad Laboratories (Cat. No: MCA5954F) | |
| TNF | MAb11 | IgG1 | BV421 | 1:100 | BioLegend (Cat. No: 502932) | |
| IFNγ | P2G10 | IgG1 | APC | 1:800 | BD Biosciences (Cat. No: 561480) | |
| IL-2 | A150D 3F1 2H2 | IgG2a | PE-Cy7 |
1:400 (primary) 1:100 (secondary) |
ThermoFisher (Cat. No: ASC0924) |
Rat-anti-mouse, IgG2a, BioLegend (Cat. No: 407114) |