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One-step RT-PCR cycling conditions for the two assays based on Fast Virus (FV) and qScript (qS) chemistries
| Cycle stage | Time (s) | Temperature ( °C) | |||
|---|---|---|---|---|---|
| qS | FV | qS | FV | ||
| Reverse transcription | 1200 | 1800 | 52.0 | 55.0 | |
| RT inactivation | 600 | 600 | 96.0 | 95.0 | |
| Denature | × 45 | 15 | 15 | 94.0 | 95.0 |
| Anneal & extension | 60 | 60 | 60.0 | 60.5 | |
