Table 3.

Oligonucleotide information and per-reaction concentration for production of the dsDNA standards using PCR.

Component	Sequence (5′ to 3′)	Concentration (nM)
Synthetic oligonucleotide*	GCTATGACCATGATTACGCCAAGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGATTCACTGGCCGTCGTTTTACA	0.4
Forward primer	GCTATGACCATGATTACGCCAA	1000
Reverse primer	TGTAAAACGACGGCCAGTGAA	1000

*The synthetic oligonucleotide was contained within a synthetic dsDNA plasmid